$124.64–$146.26
88364 is for the initial single-probe stain procedure on a specimen. Use 88365 for an additional single-probe stain procedure.
On this page
CMS RVU26D · Effective 2026-10-01
88365 In situ hybridization Medicare reimbursement rates in Virginia
Reports a manual in situ hybridization probe stain, including FISH testing on tissue or cytology specimens to identify targeted genetic changes. Compare 88365 office and facility rates across CMS payment localities in Virginia.
Amounts below use the non-QP conversion factor for participating physicians. These are base physician payments before claim-level adjustments, not patient costs or total hospital charges.
What does Medicare pay for 88365 in Virginia?
Virginia has 2 payment localities in this comparison. Use the service location to choose the applicable amount. These are the supported base rates for the 2 payment areas shown below, using the same CMS release.
Office / nonfacility
$166.35–$195.47
2 of 2 localities have a supported rate.
Facility setting
No supported rate
These are locality ranges, not averages or address-specific quotes. A facility-setting amount covers the physician service; it does not include a hospital’s separate bill. Choose a locality below to inspect its calculation, compare other payment areas, or price a percentage of Medicare.
Surgical pathology
About 88365: Manual in situ hybridization probe stain
Reports a manual in situ hybridization probe stain, including FISH testing on tissue or cytology specimens to identify targeted genetic changes.
This service covers laboratory staining and hybridization of a tissue or cytology specimen with a targeted nucleic acid probe. Fluorescence in situ hybridization (FISH) can help evaluate findings such as gene amplification or rearrangement in a tumor specimen. Pathology laboratories perform the technical work; a pathologist or other qualified professional may interpret the findings and report them. The code concerns the probe-staining procedure, not morphometric analysis of the resulting signals.
Select the code based on the documented probe-staining service performed on the specimen. The record should identify the specimen, probe or target, method, and findings supporting the test. CMS recognizes separately priced professional and technical components: report modifier 26 for interpretation and report, modifier TC for equipment and staff, or neither modifier when billing the global service.
CMS billing rules for 88365
- Professional and technical components
- Diagnostic test with a professional component (modifier 26, interpretation) and a technical component (modifier TC, equipment and staff); billing without a modifier is the global service.
Where the value comes from
- Work RVU0.86 · 17%
- Practice expense (office) RVU4.17 · 82%
- Malpractice RVU0.03 · 1%
72.2K
Medicare services in 2024 · #659 by national volume
This summary was written with AI assistance from CMS physician fee schedule data and checked against the CMS billing rules shown here. Rates on this page come directly from CMS files.
88365 compared with similar codes
Office rates for Virginia, from the same CMS release.
$261.01–$307.31
88366 reports the interpretation and report for in situ hybridization; 88365 represents probe-staining work.
$103.31–$120.56
88367 describes manual morphometric analysis of in situ hybridization results, not the probe-staining procedure.
$143.69–$168.32
88368 describes computer-assisted morphometric analysis of in situ hybridization results, rather than the stain procedure.
Compare 88365 by payment locality
Find the payment area for your service address, then open its rate page for calculation inputs, release history and the percentage-of-Medicare tool. A city may cross payment-area boundaries; the ZIP lookup above helps resolve the location.
2 of 2 payment localities
Dc + Md/Va Suburbs →
Office / nonfacility
$195.47
Facility
Unavailable
Virginia →
Office / nonfacility
$166.35
Facility
Unavailable
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88365 billing questions
How does this differ from 88364?
Both codes are in the in situ hybridization family. 88364 represents the initial single-probe stain procedure; 88365 is used for an additional single-probe stain procedure on the specimen.
Can the professional and technical work be billed separately?
Yes. Modifier 26 identifies the professional interpretation and report, while modifier TC identifies the technical work, equipment, and staff. Without either modifier, the claim represents the global service.
Does this code include interpretation and reporting?
The professional component represents interpretation and reporting. Code 88366 is the separate in situ hybridization interpretation-and-report service when that code applies.
What should the laboratory document?
Document the specimen, probe or target, staining method, and the work performed. The pathology report should support the interpretation when the professional component is billed.
When would 88367 or 88368 be more appropriate?
Those codes describe morphometric analysis of in situ hybridization results, rather than the probe-staining procedure reported with 88365.
Where these rates come from
FeeBase calculates base physician payments from CMS work, practice-expense, and malpractice RVUs, adjusted by each locality’s geographic indices and the applicable conversion factor. Unavailable or separately priced services are labeled rather than assigned a made-up amount.
Source: RVU26D. Effective 2026-10-01 through the day before 2027-01-01.
11,28588364,,Insitu hybridization (fish),A,,0.68,3.09,,3.09,NA,0.02,3.79,3.79,1,ZZZ,0.00,0.00,0.00,0,0,0,0,0,9,,33.4009,09,0,99,0.00,0.00,0.00
11,28688364,26,Insitu hybridization (fish),A,,0.68,0.26,,0.26,,0.01,0.95,0.95,1,ZZZ,0.00,0.00,0.00,0,0,0,0,0,9,,33.4009,09,0,99,0.00,0.00,0.00
11,28788364,TC,Insitu hybridization (fish),A,,0.00,2.83,,2.83,NA,0.01,2.84,2.84,1,ZZZ,0.00,0.00,0.00,0,0,0,0,0,9,,33.4009,09,0,99,0.00,0.00,0.00
11,28888365,,Insitu hybridization (fish),A,,0.86,4.17,,4.17,NA,0.03,5.06,5.06,1,XXX,0.00,0.00,0.00,0,0,0,0,0,9,,33.4009,09,0,99,0.00,0.00,0.00
11,28988365,26,Insitu hybridization (fish),A,,0.86,0.34,,0.34,,0.01,1.21,1.21,1,XXX,0.00,0.00,0.00,0,0,0,0,0,9,,33.4009,09,0,99,0.00,0.00,0.00
11,29088365,TC,Insitu hybridization (fish),A,,0.00,3.83,,3.83,NA,0.02,3.85,3.85,1,XXX,0.00,0.00,0.00,0,0,0,0,0,9,,33.4009,09,0,99,0.00,0.00,0.00
11,29188366,,Insitu hybridization (fish),A,,1.21,6.69,,6.69,NA,0.04,7.94,7.94,1,XXX,0.00,0.00,0.00,0,0,0,0,0,9,,33.4009,09,0,99,0.00,0.00,0.00