CPT code 82306: Vitamin D test, 25-hydroxy level2026 Medicare lab fee · Clinical Laboratory Fee Schedule
Measures 25-hydroxyvitamin D, the main circulating marker of vitamin D stores, in a blood specimen when clinicians assess vitamin D status.
Medicare pays $29.60 for 82306 under the 2026 Clinical Laboratory Fee Schedule: one national amount, the same in every state and setting.
Medicare lab fee · CLFS 2026 Q4
National CLFS amount
$29.60
In effect since Jan 1, 2026. The same amount in every state, payment locality and setting.
- Geographic adjustment
- None
- Office vs facility
- Same
- Since 2020
- Unchanged
- CMS updates
- Quarterly
Medicare pays the lesser of the lab’s charge and this amount. Patients usually owe no Part B deductible or coinsurance for covered clinical lab tests.
On this page 8 sections
What 82306 covers
This assay measures 25-hydroxyvitamin D in a blood specimen, typically serum. It is the main circulating marker used to assess vitamin D status and may be ordered in evaluations of suspected deficiency, bone or mineral disorders, or conditions affecting vitamin D absorption or metabolism. Primary care clinicians, endocrinologists, nephrologists, and other treating providers commonly order the test; clinical laboratories perform and report it. The measured 25-hydroxy form is distinct from the active 1,25-dihydroxy form.
Report 82306 for the 25-hydroxyvitamin D assay, including fractions when performed; do not report those fractions as separate services. Medicare pays the test only through the CLFS. The 2026 national CLFS amount is $29.60, unchanged since 2020. The amount is the same across states and localities, with no geographic or office-versus-facility adjustment. The test has no separate physician fee schedule payment.
This summary was written with AI assistance from CMS Clinical Laboratory Fee Schedule data. Amounts on this page come directly from CMS files.
How Medicare pays 82306
Clinical lab tests aren’t priced like physician services. They’re paid from their own fee schedule, with its own rules. See the whole lab fee schedule.
One national amount
CMS sets a single Clinical Laboratory Fee Schedule amount for 82306. There’s no geographic (GPCI) adjustment, so it pays the same in every state and payment locality.
No office or facility rate
Lab tests aren’t built from relative value units, so there’s no office and facility split: the place of service doesn’t change the CLFS amount.
Updated every quarter
CMS republishes the CLFS each quarter. New tests, often proprietary laboratory analyses (PLA codes), join during the year; existing amounts are updated each January.
Excluded from the physician fee schedule
The physician fee schedule lists 82306 with status X (excluded by statute), so the test itself is paid only from the CLFS.
82306 on the lab fee schedule since 2020
82306 · CLFS 2026 Q4 (current)
$29.60
Unchanged since Jan 1, 2020
27 quarterly CLFS releases on file, first CLFS 2020 Q1. A code missing from a quarter wasn’t on that release.
82306 compared with similar tests
- 82652Vitamin D1,25-dihydroxy form$38.50
- 82306 measures 25-hydroxyvitamin D, the usual marker of vitamin D status; 82652 measures the active 1,25-dihydroxy form.
- 82310Total calciumTotal, serum or plasma$5.16
- 82310 measures total calcium, not vitamin D. Report it only when a separate calcium assay is performed.
- 82330Calcium testIonized calcium$13.68
- 82330 measures ionized calcium. It is a separate calcium assay, not a vitamin D measurement.
82306 billing questions
When should 82306 be reported instead of 82652?
Use 82306 for the 25-hydroxyvitamin D measurement used to assess vitamin D status. Code 82652 measures the active 1,25-dihydroxy form and serves a different clinical question.
Are vitamin D fractions reported separately?
No. Code 82306 includes fractions when performed, so they are not separately reported as additional services.
What specimen is typically used?
The test is performed on a blood specimen, typically serum.
Is a physician interpretation separately payable?
No. Medicare pays 82306 only through the CLFS.
How does Medicare pay for 82306?
Medicare pays 82306 through the CLFS at one national amount, without a locality or office-versus-facility adjustment.
Where this amount comes from
FeeBase reads lab amounts directly from the CMS Clinical Laboratory Fee Schedule file for the quarter: one row per code, with its payment indicator and national amount. Contractor-priced tests are labeled, never given a made-up amount. Amounts are Medicare payment limits, not a patient’s bill or a commercial rate.
CMS CLFS 2026 Q4 · effective Oct 1, 2026 through the day before Jan 1, 2027
Show the CMS file line behind this amount
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