CPT code 86664: EBNA antibody, nuclear antigen target2026 Medicare lab fee · Clinical Laboratory Fee Schedule
Measures antibodies to Epstein-Barr virus nuclear antigen, commonly used with other EBV antibody results to assess whether infection is recent or past.
Medicare pays $15.29 for 86664 under the 2026 Clinical Laboratory Fee Schedule: one national amount, the same in every state and setting.
Medicare lab fee · CLFS 2026 Q4
National CLFS amount
$15.29
In effect since Jan 1, 2026. The same amount in every state, payment locality and setting.
- Geographic adjustment
- None
- Office vs facility
- Same
- Since 2020
- Unchanged
- CMS updates
- Quarterly
Medicare pays the lesser of the lab’s charge and this amount. Patients usually owe no Part B deductible or coinsurance for covered clinical lab tests.
On this page 8 sections
What 86664 covers
This serologic test measures antibodies directed against Epstein-Barr virus (EBV) nuclear antigen. It is generally performed on a blood specimen by a clinical laboratory and is often ordered with other EBV antibody tests when evaluating suspected infectious mononucleosis or clarifying a patient's EBV infection history. EBNA antibodies usually develop after the acute phase and persist, so the result is interpreted alongside other EBV markers rather than alone.
Report the test for the EBNA antibody target; distinguish it from assays for other EBV antigens. Medicare pays this laboratory service only through the CLFS. The 2026 national CLFS amount is $15.29, unchanged since 2020. The same amount applies across localities, with no office or facility payment difference. The test is excluded from the physician fee schedule.
This summary was written with AI assistance from CMS Clinical Laboratory Fee Schedule data. Amounts on this page come directly from CMS files.
How Medicare pays 86664
Clinical lab tests aren’t priced like physician services. They’re paid from their own fee schedule, with its own rules. See the whole lab fee schedule.
One national amount
CMS sets a single Clinical Laboratory Fee Schedule amount for 86664. There’s no geographic (GPCI) adjustment, so it pays the same in every state and payment locality.
No office or facility rate
Lab tests aren’t built from relative value units, so there’s no office and facility split: the place of service doesn’t change the CLFS amount.
Updated every quarter
CMS republishes the CLFS each quarter. New tests, often proprietary laboratory analyses (PLA codes), join during the year; existing amounts are updated each January.
Excluded from the physician fee schedule
The physician fee schedule lists 86664 with status X (excluded by statute), so the test itself is paid only from the CLFS.
86664 on the lab fee schedule since 2020
86664 · CLFS 2026 Q4 (current)
$15.29
Unchanged since Jan 1, 2020
27 quarterly CLFS releases on file, first CLFS 2020 Q1. A code missing from a quarter wasn’t on that release.
86664 compared with similar tests
- 86663EBV antibodyEarly antigen$13.12
- This code is specific to the EBV nuclear antigen target. Code 86663 represents a different EBV antibody assay.
- 86665VCA antibodyEpstein-Barr virus$18.14
- Use this code for antibodies to EBV viral capsid antigen; use 86664 for antibodies to EBV nuclear antigen.
- 86645CMV antibodyIgM antibody$16.85
- This code measures IgM antibody to cytomegalovirus, not an antibody to Epstein-Barr virus.
86664 billing questions
How is this different from other Epstein-Barr antibody tests?
This code identifies antibodies to EBV nuclear antigen. Use the VCA antibody code for the viral capsid antigen target and the other EBV antibody code for its distinct antibody assay.
When is EBNA antibody testing ordered?
It is commonly used with other EBV serology when evaluating suspected infectious mononucleosis or distinguishing a past EBV infection from a more recent infection.
Can it be reported with VCA antibody testing?
Yes. EBNA and VCA assays measure antibodies to different EBV targets and may be ordered and reported together as part of an EBV serologic evaluation.
What documentation supports reporting 86664?
The documentation should identify antibody testing for EBV nuclear antigen, rather than only EBV infection or a different EBV antigen.
Does Medicare pay a different amount by location or setting?
No. The CLFS amount is national and applies across localities, without an office-versus-facility payment difference.
Where this amount comes from
FeeBase reads lab amounts directly from the CMS Clinical Laboratory Fee Schedule file for the quarter: one row per code, with its payment indicator and national amount. Contractor-priced tests are labeled, never given a made-up amount. Amounts are Medicare payment limits, not a patient’s bill or a commercial rate.
CMS CLFS 2026 Q4 · effective Oct 1, 2026 through the day before Jan 1, 2027
Show the CMS file line behind this amount
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