Billing code 88350: ImmunofluorescenceMedicare rate & RVUs in Florida
Reports each additional single-antibody immunofluorescence stain performed after the first stain, such as in renal or skin biopsy evaluation.
Medicare pays $103.40–$111.46 for 88350 in the office in Florida, from Rest Of Florida to Miami. Which amount applies depends on the service address.
Medicare allowed amount for participating (non-QP) physicians, before deductible, coinsurance and claim adjustments. Not a patient’s bill or a hospital charge.
On this page 9 sections
What 88350 covers
A pathology laboratory performs an additional immunofluorescence stain to detect a specific antibody or immune reactant in tissue. The pathologist interprets the staining pattern and reports the findings. Common settings include evaluation of renal biopsy tissue for immune deposits and direct immunofluorescence studies of skin biopsies when an autoimmune blistering disorder is suspected. Laboratory staff prepare and stain the tissue; the pathologist provides the diagnostic interpretation.
Report 88350 for each additional single-antibody stain after the first stain reported with 88346. The record should identify the tissue studied, the antibodies tested, and the results supporting each stain. This is an add-on code, so it is billed with its primary procedure and paid within that procedure’s global period. Modifier 26 identifies the professional interpretation, while modifier TC identifies the technical work, equipment, and staff; reporting without either modifier represents the global service.
This summary was written with AI assistance from CMS physician fee schedule data and checked against the CMS billing rules shown here. Rates on this page come directly from CMS files.
Where 88350 pays more and less in Florida
The same RVUs, adjusted for each area’s costs. Open a locality for its calculation, history and nearby areas.
3 payment localities
$103.40 to $111.46
| Payment locality | Office | Facility |
|---|---|---|
| Fort Lauderdale | $108.55 | Unavailable |
| Miami | $111.46 | Unavailable |
| Rest Of Florida | $103.40 | Unavailable |
How the 88350 rate is calculated
Each of 88350’s three RVUs is multiplied by a geographic index (GPCI) for the payment locality, added up, and multiplied by the conversion factor. Drag the indexes or enter a ZIP to see what moves the rate.
How the rate is built · 88350
RVUs × geographic indexes × conversion factor
Work0.58
0.58 RVUs× 1.000 GPCI
Practice expense2.60
2.60 RVUs× 1.000 GPCI
Malpractice0.02
0.02 RVUs× 1.000 GPCI
Adjusted RVUs
3.2000
Conversion factor
$33.4009
Medicare rate
$106.88
Every GPCI starts at 1.000, the national rate. Enter a ZIP or drag a slider.
Payment rules and modifiers for 88350
The CMS indicators that decide how 88350 is paid alongside other services.
CMS payment indicators · 88350
Immunofluorescence
| Rule | CMS value | What it means |
|---|---|---|
| Global period | ZZZ | Add-on code: falls within the primary procedure’s global period. |
| Multiple procedures | 0 | No multiple-procedure reduction. |
| Bilateral (modifier 50) | 0 | The 150% bilateral adjustment doesn’t apply. |
| Assistant at surgery (80/81/82/AS) | 0 | Not paid without supporting documentation. |
| Co-surgeons (62) | 0 | Not permitted. |
| Team surgery (66) | 0 | Not permitted. |
| Professional/technical | 1 | Diagnostic test with separate professional (26) and technical (TC) components. |
What modifiers do to the payment
Modifier 26 · payment effect
With and without the modifier
88350 without 26 · national office
$106.88
Immunofluorescence
88350-26 · Professional component
$27.39
Pays only the interpretation and report.
88350 compared with similar codes
Compare codes · National
4 codes, side by side
How to choose
- 88346Immunofluorescence
- 88346 is for the first single-antibody immunofluorescence stain. 88350 is for each additional single-antibody stain and must be paired with a primary procedure.
- 88342Antibody stain
- 88342 reports the first immunohistochemistry or immunocytochemistry antibody stain. Choose based on the staining method documented, not merely the target antibody.
- 88344Immunostain
- 88344 describes a multiple-antibody immunohistochemistry or immunocytochemistry stain procedure; 88350 describes an additional single-antibody immunofluorescence stain.
88350 billing questions
Can 88350 be reported by itself?
No. It is an add-on for additional immunofluorescence stains and is reported with the primary stain code, 88346.
How does 88350 differ from 88346?
88346 represents the first single-antibody immunofluorescence stain. Use 88350 for each additional single-antibody stain in the same service.
When should the laboratory use an immunohistochemistry code instead?
Use the immunohistochemistry or immunocytochemistry code family when the laboratory performs that staining method rather than immunofluorescence. The documented method, not simply the antibody being tested, distinguishes the service.
Can the professional and technical portions be billed separately?
Yes. Modifier 26 identifies the pathologist’s professional interpretation, and modifier TC identifies the technical portion. Without either modifier, the claim represents the global service.
What documentation supports additional units?
Document the tissue examined and each additional antibody stain performed, with findings supporting the interpretation. Report 88350 with 88346 rather than as a standalone service.
Where these rates come from
FeeBase calculates base physician payments from CMS work, practice-expense and malpractice RVUs, adjusted by each locality’s geographic indexes and the conversion factor. Services without a published rate are labeled, never given a made-up amount. Amounts are Medicare allowed amounts before claim adjustments: not a patient’s bill or a commercial rate.
CMS RVU26D · effective Oct 1, 2026 through the day before Jan 1, 2027
Show the CMS file lines behind this rate
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