CPT code 80325: Amphetamine testing, 3 or 4 analytes2026 Medicare rate & RVUs

Medicare allowed amount for participating (non-QP) physicians, before deductible, coinsurance and claim adjustments. Not a patient’s bill or a hospital charge.

What 80325 covers

CPT 80325 identifies definitive drug testing for the amphetamine class when the assay grouping includes three or four analytes. A laboratory reports this code for testing in that analyte-count range. The distinction among 80324, 80325, and 80326 is the number of amphetamine analytes in the grouping: one or two, three or four, or five or more, respectively. Code 80307 describes presumptive drug testing using a chemistry analyzer, a different testing approach from the definitive amphetamine grouping represented by 80325.

For Medicare, status I means 80325 is not valid for Medicare reporting; Medicare uses a different code to report and pay for this service. The analyte range identifies the appropriate code in the CPT amphetamine series; it is not a count of specimens. Laboratory billing staff should distinguish this analyte grouping from presumptive drug-testing reporting, which has its own code.

This summary was written with AI assistance from CMS physician fee schedule data and checked against the CMS billing rules shown here. Rates on this page come directly from CMS files.

Where 80325 pays more and less

The same RVUs, adjusted for each area’s costs. Open a locality for its calculation, history and nearby areas.

109 of 109 payment localities

80325 rates by state

Office rate range in each state. Select a state to see its payment localities.

How the 80325 rate is calculated

Each of 80325’s three RVUs is multiplied by a geographic index (GPCI) for the payment locality, added up, and multiplied by the conversion factor. Drag the indexes or enter a ZIP to see what moves the rate.

Payment rules and modifiers for 80325

80325 has no global surgery period, no multiple-procedure, bilateral or assistant-at-surgery adjustment, and no professional/technical split. What changes the payment is where the service happens: the place of service on the claim decides whether Medicare pays the office or the facility rate.

80325 compared with similar codes

How to choose

80324Amphetamine testing1 or 2 substances

Use 80324 for amphetamine testing grouped for one or two analytes; 80325 is for three or four.

80326Amphetamine testingFive or more

Use 80326 for amphetamine testing grouped for five or more analytes; 80325 is for three or four.

80307Drug screenChemistry analyzer

80307 describes presumptive drug testing using a chemistry analyzer. 80325 represents definitive amphetamine testing for three or four analytes.

Codes related to 80325

Related codes aren’t interchangeable: bill the code that matches the service performed.

Where these rates come from

FeeBase calculates base physician payments from CMS work, practice-expense and malpractice RVUs, adjusted by each locality’s geographic indexes and the conversion factor. Services without a published rate are labeled, never given a made-up amount. Amounts are Medicare allowed amounts before claim adjustments: not a patient’s bill or a commercial rate.

CMS RVU26D · effective Oct 1, 2026 through the day before Jan 1, 2027

Open CMS sourceHow we calculate rates