CPT code 81440: Mitochondrial gene panel, at least 100 nuclear genes2026 Medicare lab fee · Clinical Laboratory Fee Schedule
Genomic analysis of at least 100 nuclear-encoded mitochondrial genes, reported for neurologic or myopathic phenotypes suggesting inherited mitochondrial disease.
Medicare pays $3,324.00 for 81440 under the 2026 Clinical Laboratory Fee Schedule: one national amount, the same in every state and setting.
Medicare lab fee · CLFS 2026 Q4
National CLFS amount
$3,324.00
In effect since Jan 1, 2026. The same amount in every state, payment locality and setting.
- Geographic adjustment
- None
- Office vs facility
- Same
- Since 2020
- Unchanged
- CMS updates
- Quarterly
Medicare pays the lesser of the lab’s charge and this amount. Patients usually owe no Part B deductible or coinsurance for covered clinical lab tests.
On this page 7 sections
What 81440 covers
This code represents genomic sequence analysis of a panel containing at least 100 nuclear-encoded genes associated with mitochondrial function. The panel is used to evaluate neurologic or myopathic phenotypes in which inherited mitochondrial disease is considered. Its gene set includes genes such as POLG, OPA1, and TK2. The analysis targets nuclear genes, not mitochondrial DNA. Molecular laboratories perform the genomic analysis, generally following an order from a clinician evaluating a patient with these features.
Medicare pays 81440 only through the Clinical Laboratory Fee Schedule (CLFS). The 2026 national CLFS amount is $3,324.00 and is unchanged from 2020 through 2026. CLFS payment is one amount per code in every state and locality, without geographic adjustment or an office-versus-facility difference. The code is not paid under the physician fee schedule.
This summary was written with AI assistance from CMS Clinical Laboratory Fee Schedule data. Amounts on this page come directly from CMS files.
How Medicare pays 81440
Clinical lab tests aren’t priced like physician services. They’re paid from their own fee schedule, with its own rules. See the whole lab fee schedule.
One national amount
CMS sets a single Clinical Laboratory Fee Schedule amount for 81440. There’s no geographic (GPCI) adjustment, so it pays the same in every state and payment locality.
No office or facility rate
Lab tests aren’t built from relative value units, so there’s no office and facility split: the place of service doesn’t change the CLFS amount.
Updated every quarter
CMS republishes the CLFS each quarter. New tests, often proprietary laboratory analyses (PLA codes), join during the year; existing amounts are updated each January.
Excluded from the physician fee schedule
The physician fee schedule lists 81440 with status X (excluded by statute), so the test itself is paid only from the CLFS.
81440 on the lab fee schedule since 2020
81440 · CLFS 2026 Q4 (current)
$3,324.00
Unchanged since Jan 1, 2020
27 quarterly CLFS releases on file, first CLFS 2020 Q1. A code missing from a quarter wasn’t on that release.
81440 compared with similar tests
- 81425Genome sequencingConstitutional or heritable disorders$5,031.20
- 81440 analyzes at least 100 nuclear-encoded genes associated with mitochondrial function; 81425 describes genome sequence analysis.
- 81441Bone marrow failure panelSequence analysis, 30 or more genes$2,448.56
- 81440 covers a panel of at least 100 nuclear-encoded mitochondrial genes. 81441 is a 30-gene IBMFS sequencing panel.
81440 billing questions
What genes does this panel assess?
It analyzes at least 100 nuclear-encoded genes associated with mitochondrial function, including genes such as POLG, OPA1, and TK2.
How does 81440 differ from mitochondrial DNA testing?
81440 analyzes nuclear-encoded genes associated with mitochondrial function; it does not analyze the mitochondrial genome itself.
How does 81440 differ from 81425?
81440 is a panel focused on nuclear-encoded mitochondrial genes. 81425 describes genome sequence analysis.
How does Medicare pay for 81440?
Medicare pays it through the CLFS only, at one national amount across localities and without an office-versus-facility difference.
Where this amount comes from
FeeBase reads lab amounts directly from the CMS Clinical Laboratory Fee Schedule file for the quarter: one row per code, with its payment indicator and national amount. Contractor-priced tests are labeled, never given a made-up amount. Amounts are Medicare payment limits, not a patient’s bill or a commercial rate.
CMS CLFS 2026 Q4 · effective Oct 1, 2026 through the day before Jan 1, 2027
Show the CMS file line behind this amount
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