CPT code 84140: Pregnenolone, reproductive hormone assay2026 Medicare lab fee · Clinical Laboratory Fee Schedule
Measures pregnenolone, a reproductive hormone, and is reported when a laboratory order calls for measurement of this specific analyte.
Medicare pays $20.67 for 84140 under the 2026 Clinical Laboratory Fee Schedule: one national amount, the same in every state and setting.
Medicare lab fee · CLFS 2026 Q4
National CLFS amount
$20.67
In effect since Jan 1, 2026. The same amount in every state, payment locality and setting.
- Geographic adjustment
- None
- Office vs facility
- Same
- Since 2020
- Unchanged
- CMS updates
- Quarterly
Medicare pays the lesser of the lab’s charge and this amount. Patients usually owe no Part B deductible or coinsurance for covered clinical lab tests.
On this page 7 sections
What 84140 covers
This laboratory test measures pregnenolone, a reproductive hormone. It is reported when pregnenolone is the analyte ordered. It is distinct from the assay for 17-hydroxypregnenolone (84143) and the progesterone assay (84144); those codes identify different analytes, not alternate names for pregnenolone. Laboratories report the measured pregnenolone result.
Report 84140 for the pregnenolone assay performed. Medicare pays it through the CLFS only. CMS sets one national amount per code that applies in every state and locality, with no geographic adjustment or office-versus-facility difference. The 2026 national CLFS amount is $20.67, unchanged since 2020. The test is excluded from the physician fee schedule. Medicare recorded 35,287 office services and no facility services in 2024.
This summary was written with AI assistance from CMS Clinical Laboratory Fee Schedule data. Amounts on this page come directly from CMS files.
How Medicare pays 84140
Clinical lab tests aren’t priced like physician services. They’re paid from their own fee schedule, with its own rules. See the whole lab fee schedule.
One national amount
CMS sets a single Clinical Laboratory Fee Schedule amount for 84140. There’s no geographic (GPCI) adjustment, so it pays the same in every state and payment locality.
No office or facility rate
Lab tests aren’t built from relative value units, so there’s no office and facility split: the place of service doesn’t change the CLFS amount.
Updated every quarter
CMS republishes the CLFS each quarter. New tests, often proprietary laboratory analyses (PLA codes), join during the year; existing amounts are updated each January.
Excluded from the physician fee schedule
The physician fee schedule lists 84140 with status X (excluded by statute), so the test itself is paid only from the CLFS.
84140 on the lab fee schedule since 2020
84140 · CLFS 2026 Q4 (current)
$20.67
Unchanged since Jan 1, 2020
27 quarterly CLFS releases on file, first CLFS 2020 Q1. A code missing from a quarter wasn’t on that release.
84140 compared with similar tests
- 84143Steroid assay17-hydroxypregnenolone level$22.81
- Use 84143 for 17-hydroxypregnenolone; use 84140 for pregnenolone itself.
- 84144Progesterone testSerum concentration$20.86
- Code 84144 identifies the progesterone assay, a different analyte from pregnenolone.
- 84135PregnanediolReproductive hormone level$21.27
- Code 84135 identifies the pregnanediol assay, not a pregnenolone assay.
84140 billing questions
When should 84140 be reported instead of 84143?
Report 84140 for pregnenolone. Code 84143 identifies the assay for 17-hydroxypregnenolone.
Is 84140 the same test as a progesterone assay?
No. Pregnenolone and progesterone are different analytes; 84144 identifies the progesterone assay.
How many units should be reported?
Report the assay performed. Do not use multiple units to represent the measured concentration.
Does Medicare pay 84140 under the physician fee schedule?
No. Medicare pays this test through the CLFS, with one national amount across localities and no office-versus-facility payment difference.
Where this amount comes from
FeeBase reads lab amounts directly from the CMS Clinical Laboratory Fee Schedule file for the quarter: one row per code, with its payment indicator and national amount. Contractor-priced tests are labeled, never given a made-up amount. Amounts are Medicare payment limits, not a patient’s bill or a commercial rate.
CMS CLFS 2026 Q4 · effective Oct 1, 2026 through the day before Jan 1, 2027
Show the CMS file line behind this amount
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