CPT code 84143: Steroid assay, 17-hydroxypregnenolone level2026 Medicare lab fee · Clinical Laboratory Fee Schedule
Measures 17-hydroxypregnenolone, a steroid hormone precursor, during evaluation of adrenal steroid production and suspected congenital adrenal hyperplasia.
Medicare pays $22.81 for 84143 under the 2026 Clinical Laboratory Fee Schedule: one national amount, the same in every state and setting.
Medicare lab fee · CLFS 2026 Q4
National CLFS amount
$22.81
In effect since Jan 1, 2026. The same amount in every state, payment locality and setting.
- Geographic adjustment
- None
- Office vs facility
- Same
- Since 2020
- Unchanged
- CMS updates
- Quarterly
Medicare pays the lesser of the lab’s charge and this amount. Patients usually owe no Part B deductible or coinsurance for covered clinical lab tests.
On this page 7 sections
What 84143 covers
This assay measures 17-hydroxypregnenolone, an intermediate in adrenal and gonadal steroid production, typically in a serum specimen. Endocrinologists and other clinicians may order it when evaluating abnormal adrenal steroid synthesis, including suspected enzyme defects such as 3-beta-hydroxysteroid dehydrogenase deficiency. Clinical laboratories perform and report the measurement, sometimes as part of a broader steroid evaluation.
Report the assay for the 17-hydroxypregnenolone determination; it is distinct from assays of related steroid hormones or metabolites. Medicare pays this test through the CLFS, with one national amount across states and localities and no office-versus-facility payment difference. The 2026 national amount is $22.81, unchanged since 2020. The test is excluded from the physician fee schedule and paid only through the CLFS.
This summary was written with AI assistance from CMS Clinical Laboratory Fee Schedule data. Amounts on this page come directly from CMS files.
How Medicare pays 84143
Clinical lab tests aren’t priced like physician services. They’re paid from their own fee schedule, with its own rules. See the whole lab fee schedule.
One national amount
CMS sets a single Clinical Laboratory Fee Schedule amount for 84143. There’s no geographic (GPCI) adjustment, so it pays the same in every state and payment locality.
No office or facility rate
Lab tests aren’t built from relative value units, so there’s no office and facility split: the place of service doesn’t change the CLFS amount.
Updated every quarter
CMS republishes the CLFS each quarter. New tests, often proprietary laboratory analyses (PLA codes), join during the year; existing amounts are updated each January.
Excluded from the physician fee schedule
The physician fee schedule lists 84143 with status X (excluded by statute), so the test itself is paid only from the CLFS.
84143 on the lab fee schedule since 2020
84143 · CLFS 2026 Q4 (current)
$22.81
Unchanged since Jan 1, 2020
27 quarterly CLFS releases on file, first CLFS 2020 Q1. A code missing from a quarter wasn’t on that release.
84143 compared with similar tests
- 84140PregnenoloneReproductive hormone assay$20.67
- Use 84140 for pregnenolone itself. Use 84143 when the measured analyte is 17-hydroxypregnenolone.
- 8349817-OHP test$27.17
- 83498 measures 17-hydroxyprogesterone, not 17-hydroxypregnenolone. The analytes are related but are not interchangeable.
- 84138PregnanetriolUrinary steroid metabolite$21.05
- 84138 measures pregnanetriol, a different steroid metabolite. Choose 84143 for the 17-hydroxypregnenolone assay.
84143 billing questions
How is this different from 17-hydroxyprogesterone testing?
This assay measures 17-hydroxypregnenolone, a different steroid precursor. CPT 83498 measures 17-hydroxyprogesterone, commonly assessed in the evaluation of congenital adrenal hyperplasia.
How does it differ from the pregnenolone assay?
CPT 84140 measures pregnenolone, while 84143 measures 17-hydroxypregnenolone. Select the code for the specific analyte tested.
What specimen is typically tested?
The assay is typically performed on serum. Follow the performing laboratory's specimen instructions for collection and handling.
Does Medicare pay a separate physician interpretation?
The test is excluded from the physician fee schedule and is paid only through the CLFS.
How is Medicare payment determined?
CMS sets one national CLFS amount for the code. The amount applies across localities without an office or facility difference.
Where this amount comes from
FeeBase reads lab amounts directly from the CMS Clinical Laboratory Fee Schedule file for the quarter: one row per code, with its payment indicator and national amount. Contractor-priced tests are labeled, never given a made-up amount. Amounts are Medicare payment limits, not a patient’s bill or a commercial rate.
CMS CLFS 2026 Q4 · effective Oct 1, 2026 through the day before Jan 1, 2027
Show the CMS file line behind this amount
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