CPT code 86325: Immunoelectrophoresis, concentrated nonserum fluids2026 Medicare lab fee · Clinical Laboratory Fee Schedule
Immunoelectrophoresis of concentrated body fluids, such as urine or cerebrospinal fluid, is reported when this method is performed on a nonserum specimen.
Medicare pays $23.13 for 86325 under the 2026 Clinical Laboratory Fee Schedule: one national amount, the same in every state and setting.
Medicare lab fee · CLFS 2026 Q4
National CLFS amount
$23.13
In effect since Jan 1, 2026. The same amount in every state, payment locality and setting.
- Geographic adjustment
- None
- Office vs facility
- Same
- Since 2020
- Unchanged
- CMS updates
- Quarterly
Medicare pays the lesser of the lab’s charge and this amount. Patients usually owe no Part B deductible or coinsurance for covered clinical lab tests.
On this page 8 sections
What 86325 covers
This laboratory analysis uses immunoelectrophoresis to separate and assess proteins in a concentrated body fluid other than serum. Urine and cerebrospinal fluid are examples. Report this code when the method is performed on such a specimen; the fluid context distinguishes it from serum immunoelectrophoresis. A clinical laboratory performs the analytic test, and a physician may provide a separate interpretation. This is an immunoelectrophoresis test, not an immunofixation method.
Medicare pays the laboratory test through the CLFS. The 2026 national CLFS amount is $23.13, unchanged from 2020 through 2026. One amount applies in every locality, with no office or facility difference. A separate physician interpretation is payable under the physician fee schedule when billed with modifier 26.
This summary was written with AI assistance from CMS Clinical Laboratory Fee Schedule data. Amounts on this page come directly from CMS files.
How Medicare pays 86325
Clinical lab tests aren’t priced like physician services. They’re paid from their own fee schedule, with its own rules. See the whole lab fee schedule.
One national amount
CMS sets a single Clinical Laboratory Fee Schedule amount for 86325. There’s no geographic (GPCI) adjustment, so it pays the same in every state and payment locality.
No office or facility rate
Lab tests aren’t built from relative value units, so there’s no office and facility split: the place of service doesn’t change the CLFS amount.
Updated every quarter
CMS republishes the CLFS each quarter. New tests, often proprietary laboratory analyses (PLA codes), join during the year; existing amounts are updated each January.
Excluded from the physician fee schedule
The physician fee schedule lists 86325 with status X (excluded by statute), so the test itself is paid only from the CLFS.
Physician interpretation (modifier 26)
A physician’s interpretation is billed separately with modifier 26 and paid under the physician fee schedule: $17.70 nationally in the office, before locality adjustment.
86325 on the lab fee schedule since 2020
86325 · CLFS 2026 Q4 (current)
$23.13
Unchanged since Jan 1, 2020
27 quarterly CLFS releases on file, first CLFS 2020 Q1. A code missing from a quarter wasn’t on that release.
86325 compared with similar tests
- 86320ImmunoelectrophoresisSerum$29.92
- Choose 86320 when the immunoelectrophoresis specimen is serum; choose 86325 for another concentrated body fluid, such as urine or cerebrospinal fluid.
- 86335ImmunofixationConcentrated urine or CSF$29.35
- 86335 identifies immunofixation electrophoresis for urine or cerebrospinal fluid. Code 86325 is for immunoelectrophoresis of other fluids.
- 86334ImmunofixationSerum specimen$22.34
- 86334 is serum immunofixation electrophoresis, a different method and specimen context from immunoelectrophoresis of a concentrated nonserum fluid.
86325 billing questions
When should 86325 be used instead of 86320?
Use 86325 for immunoelectrophoresis of a concentrated nonserum body fluid, such as urine or cerebrospinal fluid. Use 86320 for serum immunoelectrophoresis.
Is a physician interpretation separately reportable?
A separate physician interpretation is payable under the physician fee schedule when billed with modifier 26. The laboratory test itself is paid under the CLFS.
How does Medicare pay for the laboratory test?
Medicare pays one national CLFS amount per code, with no locality or office-versus-facility adjustment.
How does 86325 differ from urine or CSF immunofixation?
86325 describes immunoelectrophoresis of another fluid. Code 86335 describes immunofixation electrophoresis for urine or cerebrospinal fluid, a different method.
Where this amount comes from
FeeBase reads lab amounts directly from the CMS Clinical Laboratory Fee Schedule file for the quarter: one row per code, with its payment indicator and national amount. Contractor-priced tests are labeled, never given a made-up amount. Amounts are Medicare payment limits, not a patient’s bill or a commercial rate.
CMS CLFS 2026 Q4 · effective Oct 1, 2026 through the day before Jan 1, 2027
Show the CMS file line behind this amount
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