CPT code 89329: Hamster penetration, sperm function assay2026 Medicare lab fee · Clinical Laboratory Fee Schedule
This specialized male-fertility assay assesses sperm penetration of hamster oocytes and is reported when that functional evaluation is performed.
Medicare pays $19.59 for 89329 under the 2026 Clinical Laboratory Fee Schedule: one national amount, the same in every state and setting.
Medicare lab fee · CLFS 2026 Q4
National CLFS amount
$19.59
In effect since Jan 1, 2026. The same amount in every state, payment locality and setting.
- Geographic adjustment
- None
- Office vs facility
- Same
- Since 2020
- Unchanged
- CMS updates
- Quarterly
Medicare pays the lesser of the lab’s charge and this amount. Patients usually owe no Part B deductible or coinsurance for covered clinical lab tests.
On this page 8 sections
What 89329 covers
This specialized andrology test assesses sperm function by measuring the ability of sperm in a semen specimen to penetrate hamster oocytes. It may be ordered as part of an infertility evaluation when the clinical question concerns sperm function beyond routine semen characteristics such as count or motility. A reproductive laboratory or andrology laboratory performs and reports the assay.
Report 89329 for the penetration assay itself, not for a routine semen analysis or sperm antibody testing. The 2026 national CLFS amount is $19.59, unchanged since 2020. Medicare pays one amount per code nationwide, without locality or office-versus-facility adjustment; the test is paid only through the CLFS.
This summary was written with AI assistance from CMS Clinical Laboratory Fee Schedule data. Amounts on this page come directly from CMS files.
How Medicare pays 89329
Clinical lab tests aren’t priced like physician services. They’re paid from their own fee schedule, with its own rules. See the whole lab fee schedule.
One national amount
CMS sets a single Clinical Laboratory Fee Schedule amount for 89329. There’s no geographic (GPCI) adjustment, so it pays the same in every state and payment locality.
No office or facility rate
Lab tests aren’t built from relative value units, so there’s no office and facility split: the place of service doesn’t change the CLFS amount.
Updated every quarter
CMS republishes the CLFS each quarter. New tests, often proprietary laboratory analyses (PLA codes), join during the year; existing amounts are updated each January.
Excluded from the physician fee schedule
The physician fee schedule lists 89329 with status X (excluded by statute), so the test itself is paid only from the CLFS.
89329 on the lab fee schedule since 2020
89329 · CLFS 2026 Q4 (current)
$19.59
Unchanged since Jan 1, 2020
27 quarterly CLFS releases on file, first CLFS 2020 Q1. A code missing from a quarter wasn’t on that release.
89329 compared with similar tests
- 89310Semen analysisMotility and count$8.61
- 89310 represents semen analysis with sperm count. Choose 89329 for the specialized hamster-oocyte penetration test, not a count result.
- 89320Semen analysisVolume, count, motility, differential$12.31
- 89320 covers semen volume, count, and motility measurements. 89329 evaluates sperm penetration of hamster oocytes.
- 89325Sperm antibody$10.67
- 89325 is used for sperm antibody testing. 89329 assesses sperm penetration function rather than antibodies.
- 89300Semen analysisIncludes postcoital testing$9.84
- 89300 is a semen-analysis service associated with postcoital testing. 89329 identifies the hamster-oocyte penetration assay.
89329 billing questions
When should 89329 be selected instead of a routine semen analysis?
Use 89329 when the performed test evaluates sperm penetration of hamster oocytes. Routine sperm count, volume, and motility testing is represented by other semen-analysis codes.
Does 89329 include sperm count or motility testing?
The code identifies the hamster-oocyte penetration assay, not routine semen parameters. Report another semen-analysis service only when that work is separately performed and documented.
What specimen is used for this test?
The assay evaluates sperm from a semen specimen against hamster oocytes.
Is modifier 26 appropriate for 89329?
No. Medicare pays this test only through the CLFS; it has no separate physician-fee-schedule payment.
Does Medicare pay 89329 through the physician fee schedule?
No. The test is paid only through the CLFS.
Where this amount comes from
FeeBase reads lab amounts directly from the CMS Clinical Laboratory Fee Schedule file for the quarter: one row per code, with its payment indicator and national amount. Contractor-priced tests are labeled, never given a made-up amount. Amounts are Medicare payment limits, not a patient’s bill or a commercial rate.
CMS CLFS 2026 Q4 · effective Oct 1, 2026 through the day before Jan 1, 2027
Show the CMS file line behind this amount
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